Compatibility and Correlation
Accurate quantification of target antigens in a human sample can be achieved with any type of chemiluminescence detection in the All-in-One immunoassay, provided that a specific antibody conjugated with a label exhibits high selectivity and rapidly binds with the target antigen, as illustrated in the third and fourth slides.
In the final step, the kit emits bright light when the label-conjugated antibody bound to the target antigen reacts with chemiluminescence reagents, as depicted in the fifth slide. The color of chemiluminescence emitted in the All-in-One immunoassay depends on the properties of the label-conjugated antibody and/or reagents.
We possess both primitive and advanced technology related to the 1,1'-Oxalyldiimidazole (ODI) and 3,4,5-trimethoxylphenylglyoxal hydrate (TMPG) chemiluminescence detections, which have been applied in the All-in-One immunoassays. These chemiluminescence detections are superior to or comparable with conventional chemiluminescence detections, such as acridinium ester, AMPPD, and luminol chemiluminescence detections.
The detection methods employed in the All-in-One immunoassay are compatible with those used in conventional immunoassays. As a result, the All-in-One immunoassay can be utilized with various detection methods, including bioluminescence, chemiluminescence, colorimetric, electrochemiluminescence, electrochemical, naked-eye, and radioactivity.