Principle of All-in-One Immunoassay
Moreover, the All-in-One immunoassay offers superior sensitivity as the products formed during the immunoreaction are in a solution phase. In contrast, conventional immunoassays immobilize the reaction products on the surface of a solid substrate, thereby limiting their interaction with detection reagents. As a result, the products in the All-in-One immunoassay readily and quickly react with chemiluminescent (CL) reagents, producing bright light emissions. On the other hand, products in the conventional immunoassays react less efficiently with CL reagents and generate relatively dim light. These advantages make the All-in-One immunoassay a promising tool for research and diagnostic testing. However, further studies are needed to assess its specificity, limitations, and potential applications.