The slideshow above demonstrates that various chemiluminescence detections are utilized in the All-in-One immunoassay. To measure chemiluminescence in the All-in-One immunoassay, a kit should include antibodies that are conjugated with a specific label capable of emitting chemiluminescence, as shown in the second slide.
Accurate quantification of target antigens in a human sample can be achieved with any type of chemiluminescence detection in the All-in-One immunoassay, provided that a specific antibody conjugated with a label exhibits high selectivity and rapidly binds with the target antigen, as illustrated in the third and fourth slides.
In the final step, the kit emits bright light when the label-conjugated antibody bound to the target antigen reacts with chemiluminescence reagents, as depicted in the fifth slide. The color of chemiluminescence emitted in the All-in-One immunoassay depends on the properties of the label-conjugated antibody and/or reagents.
We possess both primitive and advanced technology related to the 1,1'-Oxalyldiimidazole (ODI) and 3,4,5-trimethoxylphenylglyoxal hydrate (TMPG) chemiluminescence detections, which have been applied in the All-in-One immunoassays. These chemiluminescence detections are superior to or comparable with conventional chemiluminescence detections, such as acridinium ester, AMPPD, and luminol chemiluminescence detections.
The detection methods employed in the All-in-One immunoassay are compatible with those used in conventional immunoassays. As a result, the All-in-One immunoassay can be utilized with various detection methods, including bioluminescence, chemiluminescence, colorimetric, electrochemiluminescence, electrochemical, naked-eye, and radioactivity.
Accurate quantification of target antigens in a human sample can be achieved with any type of chemiluminescence detection in the All-in-One immunoassay, provided that a specific antibody conjugated with a label exhibits high selectivity and rapidly binds with the target antigen, as illustrated in the third and fourth slides.
In the final step, the kit emits bright light when the label-conjugated antibody bound to the target antigen reacts with chemiluminescence reagents, as depicted in the fifth slide. The color of chemiluminescence emitted in the All-in-One immunoassay depends on the properties of the label-conjugated antibody and/or reagents.
We possess both primitive and advanced technology related to the 1,1'-Oxalyldiimidazole (ODI) and 3,4,5-trimethoxylphenylglyoxal hydrate (TMPG) chemiluminescence detections, which have been applied in the All-in-One immunoassays. These chemiluminescence detections are superior to or comparable with conventional chemiluminescence detections, such as acridinium ester, AMPPD, and luminol chemiluminescence detections.
The detection methods employed in the All-in-One immunoassay are compatible with those used in conventional immunoassays. As a result, the All-in-One immunoassay can be utilized with various detection methods, including bioluminescence, chemiluminescence, colorimetric, electrochemiluminescence, electrochemical, naked-eye, and radioactivity.
.jpg)
Fig. 1 Various detection methods applied for the All-in-One immunoassay for the rapid quantification of trace D-dimer (2 ng/ml) in human serum.
.jpg)
Fig. 2 Improvement of acridinium ester cheniluminescence detection method.
As demonstrated in Fig. 1, the All-in-One immunoassay utilizing bio- and chemi-luminescence detections can accurately quantify trace levels of D-dimer (as low as 2 ng/ml) in human serum in less than 10 minutes. Luminescent MD has also developed various techniques to enhance the sensitivity of various chemiluminescence detections. For instance, a new acridinium ester chemiluminescence reagent has been created, resulting in much brighter chemiluminescence emission with the advanced detection system than the current detection method, as depicted in Fig. 2. The All-in-One immunoassay employing the new CL reagents for acridinium ester chemiluminescence is substantially more sensitive than the previous CL reagents commonly used in the field. We are developing portable or laboratory-based devices and kits based on the unique characteristics of each detection method.
.jpg)
Fig. 3 Correlations of chemilumnescence detection methods in the All-in-One immunoassay for the rapid quantification of troponin I in human serum.
The All-in-One immunoassay utilizing chemiluminescence detection has been validated as a novel diagnostic tool for early detection of cardiac conditions, including heart attack and myocardial infarction. In particular, the All-in-One immunoassay can accurately and precisely quantify trace levels of troponin I, the biomarker of choice for detecting cardiac injury in patients with renal failure, in human serum within 6 to 10 minutes. This has been demonstrated using three different chemiluminescence detections, namely Acridinium ester, luminol, and AMPPD chemiluminescence detections. The excellent correlation between these three different chemiluminescence detections presented in Fig. 3 confirms that they are highly sensitive detection methods suitable for use in the All-in-One immunoassay.
Fig. 4 Applications of the All-in-One immunoassay with excellent compatibility and correlation.
As shown in Fig. 4, the All-in-One immunoassay has the potential to replace traditional immunoassays and become widely used in healthcare. One major advantage of the All-in-One immunoassay is its ability to utilize any type of chemiluminescence detection. This feature enables the All-in-One immunoassay to be seamlessly integrated into diagnostic devices already commercialized by leading companies such as Abbott, Roche, and Siemens Healthneer without requiring any modifications. This opens up possibilities for Luminescent MD to form various collaborations with these leading companies and rapidly grow their business related to the All-in-One immunoassay.
For more detailed information on the characteristics, detection methods, diagnostic kits, devices, and other related aspects of the "All-In-One Immunoassay," please refer to the separate pages on our website. Additionally, important information is organized in our FAQs page for your convenience.