Scheme 1. Bioluminescence of antigen-binding antibodies conjugated with luciferase formed in an all-in-one immunoassay.
Scheme 2. Bioluminescence of an antibody-binding antibody conjugated with a luciferin derivative, such as (a) 6-amino-D-luciferin and (b) 6-aminoseleno-D-luciferin, formed an all-in-one immunoassay.